Welcome to the Calo Lab!

Research

We study the molecular entities controlling and coordinating RNA metabolism, the compendium of all processes that involve RNA, such as synthesis, processing, modifications, export, translation, and degradation, and their role in development and disease. A fundamental property of the cell is its capacity to simultaneously modulate all aspects of RNA metabolism in space and time, across different cellular compartments and in response to environmental signals. However, it is not clear how the cell achieves such a broad array of functions. Thus, the goal of our research program is to decipher how different aspects of RNA metabolism are coordinately controlled to generate structure and function during development and how mutations in components of the RNA metabolic program lead to congenital disorders and cancer. We use disease-relevant animal models to identify mechanisms and pathways by which alterations in RNA metabolism lead to disease, and a combination of genetic, biochemical, and functional genomic approaches to establish the cellular functions of genes in which mutations cause RNA metabolic disorders. We envision that our work will provide unique insights into the structure and function of our cell-type diversity and potential opportunities for therapeutic interventions of a broader spectrum of complex human disorders.

People

Eliezer Calo-Velazquez

Eliezer Calo-Velazquez

Principal Investigator

calo#mit.edu

Cindy Woolley

Cindy Woolley

Administrative Assistant

cwoolley#mit.edu

Jialiang Liang

Jialiang Liang

Postdoctoral Associate

jlliang#mit.edu

Arish Shah

Arish Shah

Graduate Student

arish#mit.edu

Byron Lee

Byron Lee

Graduate Student

byronlee#mit.edu

Nima Jaberi-Lashkari

Nima Jaberi-Lashkari

Graduate Student

njaberi#mit.edu

Patrick Aoude

Patrick Aoude

Research Support Associate

aoude#mit.edu

Fardin Aryan

Fardin Aryan

Research Support Associate

fardin#mit.edu

Publications

  • Flynn, RA, Do, BT, Rubin, AJ, Calo, E, Lee, B, Kuchelmeister, H et al.. 7SK-BAF axis controls pervasive transcription at enhancers. Nat. Struct. Mol. Biol. 2016;23 (3):231-8. doi: 10.1038/nsmb.3176. PubMed PMID:26878240 PubMed Central PMC4982704.

  • Calo, E, Flynn, RA, Martin, L, Spitale, RC, Chang, HY, Wysocka, J et al.. RNA helicase DDX21 coordinates transcription and ribosomal RNA processing. Nature. 2015;518 (7538):249-53. doi: 10.1038/nature13923. PubMed PMID:25470060 PubMed Central PMC4827702.

  • Buecker, C, Srinivasan, R, Wu, Z, Calo, E, Acampora, D, Faial, T et al.. Reorganization of enhancer patterns in transition from naive to primed pluripotency. Cell Stem Cell. 2014;14 (6):838-53. doi: 10.1016/j.stem.2014.04.003. PubMed PMID:24905168 PubMed Central PMC4491504.

  • Hilgendorf, KI, Leshchiner, ES, Nedelcu, S, Maynard, MA, Calo, E, Ianari, A et al.. The retinoblastoma protein induces apoptosis directly at the mitochondria. Genes Dev. 2013;27 (9):1003-15. doi: 10.1101/gad.211326.112. PubMed PMID:23618872 PubMed Central PMC3656319.

  • Calo, E, Wysocka, J. Modification of enhancer chromatin: what, how, and why? Mol. Cell. 2013;49 (5):825-37. doi: 10.1016/j.molcel.2013.01.038. PubMed PMID:23473601 PubMed Central PMC3857148.

  • Calo, E, Quintero-Estades, JA, Danielian, PS, Nedelcu, S, Berman, SD, Lees, JA et al.. Rb regulates fate choice and lineage commitment in vivo. Nature. 2010;466 (7310):1110-4. doi: 10.1038/nature09264. PubMed PMID:20686481 PubMed Central PMC2933655.

  • Pritchard, CD, O’Shea, TM, Siegwart, DJ, Calo, E, Anderson, DG, Reynolds, FM et al.. An injectable thiol-acrylate poly(ethylene glycol) hydrogel for sustained release of methylprednisolone sodium succinate. Biomaterials. 2011;32 (2):587-97. doi: 10.1016/j.biomaterials.2010.08.106. PubMed PMID:20880573 PubMed Central PMC4070531.

  • Ianari, A, Natale, T, Calo, E, Ferretti, E, Alesse, E, Screpanti, I et al.. Proapoptotic function of the retinoblastoma tumor suppressor protein. Cancer Cell. 2009;15 (3):184-94. doi: 10.1016/j.ccr.2009.01.026. PubMed PMID:19249677 PubMed Central PMC2880703.

  • Berman, SD, Calo, E, Landman, AS, Danielian, PS, Miller, ES, West, JC et al.. Metastatic osteosarcoma induced by inactivation of Rb and p53 in the osteoblast lineage. Proc. Natl. Acad. Sci. U.S.A. 2008;105 (33):11851-6. doi: 10.1073/pnas.0805462105. PubMed PMID:18697945 PubMed Central PMC2575280.